This page serves as a web portal to access our datasets of translational profiling of microglia. Please cite Kang et al (http://jem.rupress.org/content/early/2018/08/03/jem.20180653.full). Although more data will be added in the future, the following datasets are available for query for your gene of interest:
Male middle/ young: 12 vs 3 month old male
Male old/young: 24 vs 3 month old male
Female middle/ young: 12 vs 3 month old female
Female old/young: 24 vs 3 month old female
Old female/old male: 24 month old female vs 24 month old male
Amyloidosis: APP/PS1 mouse model vs wild-type littermates at 9 months of age
Tauopathy: P0 injection of AAV-Tau-P301L vs AAV-GFP controls at 9 months of age
Lipopolysaccharide (LPS): 24 hours post-ip injection of 2mg/kg LPS vs saline controls
Polyinosinic:polycytidylic (Poly(I:C)): 24 hours post-ip injection of 12 mg/kg pIC vs saline controls
Sort/RiboTag: Microglia sorted by CD11b+;CD45-lo/int vs RiboTag method
To begin you query, please enter the web portal powered by Shiny Apps at https://microglia.shinyapps.io/Shiny_RiboTag/
The Excel file containing streamlined RiboTag data can be downloaded here. This file is group averages of RPKM of protein coding genes after removal of zero or lowly expressed genes (log2 RPKM < -2).
The larger Excel file containing RiboTag data can be downloaded from GEO repository at https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117646
NOTE: Please bookmark this page since the actual hosting of this data may change over time (but this page will update to reflect any new links/portals).